Irradiated Herpes Simplex Virus
نویسنده
چکیده
Aphidicolin is a potent inhibitor of both host cell DNA polymerase a and herpes simplex virus (HSV)induced DNA polymerase but has no effect on DNA polymerases ,B and y of host cells. By using an aphidicolin-resistant mutant (Aphr) of HSV, a possible involvement of DNA polymerase a in host cell reactivation of UV-damaged HSV was studied. Plaque formation by UV-irradiated Aph' was markedly inhibited by 1 [Lg of aphidicolin per ml, which did not affect the plating efficiency of nonirradiated Aphr. Aphidicolin added before 12 h postinfection inhibited plaque formation by irradiated Aphr, which became aphidicolin insensitive after 36 h postinfection. The results strongly suggest that host cell DNA polymerase a is involved in the repair of UV-irradiated HSV DNA.
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